Pathoscreen® kits for the diagnosis of plant pathogens are ELISA test kits that contain all the materials necessary to perform an ELISA protocol. The kits include precoated plates which save time and offer extraordinary stability. Kits are available in three sizes, 96, 288, and 480 test wells. ELISA kits are perfect to perform high-throughput tests.
VegDr provides up-to-date information on vegetable diseases (in Georgia). The app contains information on various diseases, images of the symptoms, and lists the products that are considered effective for control. At the present time, the app includes cucurbits (cucumber, watermelon, squash, cantaloupe, pumpkin) and solanaceous crops (tomato and pepper).
DAS-ELISA (Double Antibody Sandwich ELISA) is a double antibody sandwich enzyme-linked immunosorbent method for rapid detection of virus infection in different crops (tomato, lettuce, onion, citrus, pepper, etc).
The test can be used for rapid detection of CTV (citrus tristeza virus) in various tissues of citrus. Field samples should be collected preferably in spring or autumn, whereas sampling during prolonged hot weather should be avoided. Samples from 3-4 different locations on the tree should be pooled and samples are homogenized 1:20 (w/v) in an extraction buffer (Garnsey & Cambra (1991).
Qualiplante and BIOREBA provide PCR and qPCR genetic detection products. These sets are ready-to-use, optimized for the application, validated (sensitivity, specificity, repeatability, reproducibility) and have lot-to-lot consistency. A Certificate of Analysis (CoA) is include included. One-step-reactions are used and positive and negative controls are included.
BIOREBA AG is an independent biotech company providing diagnostic tests for the detection of plant pathogens. The company supplies products for testing potatoes, vines, fruit trees, small fruits, vegetables, ornamental plants, and field crops.
The rapid one-step assay AgriStrip, developed and manufactured by BIOREBA, is based on lateral flow immunochromatography. The AgriStrip test has been developed to confirm the presence of a plant pathogen in samples with suspicious symptoms.
The kits contain a thermostable Reverse Transcriptase and a Hot-Start DNA Polymerase, pathogen specific primers and dNTPs, DNA-based positive and negative controls for the PCR reaction. The reaction is carried out in one tube starting with the reverse transcription of virus RNA and subsequent cDNA amplification.The amplicon can be visualized on a standard agarose gel. Each kit is provided with detailed instructions and product specifications and quality validation data. Please note that reagents for RNA isolation are not included with this kit.
LOEWE's DNA PCR sets contain a premix, including the pathogen-specific primers and dNTPs for 100 PCR reactions and positive and negative controls for 10 reactions. All components are pre-tested and each product is accompanied by a lot-specific certificate and a working protocol.
The LOEWE®FAST rapid test series allows reliable and specific detection of plant pathogens within minutes. As stand-alone diagnostic tools, these tests provide a quick and easy assessment of suspicious plant material in the field or greenhouse without the need for a laboratory.