The kits contain a thermostable Reverse Transcriptase and a Hot-Start DNA Polymerase, pathogen specific primers and dNTPs, DNA-based positive and negative controls for the PCR reaction. The reaction is carried out in one tube starting with the reverse transcription of virus RNA and subsequent cDNA amplification.The amplicon can be visualized on a standard agarose gel. Each kit is provided with detailed instructions and product specifications and quality validation data. Please note that reagents for RNA isolation are not included with this kit.
Laboratory equipment for PCR
Specialist training needed
Depends on the kit size and amount