DAS-ELISA
DAS-ELISA
entity.ipm.label.technical_specification
Raven tehnološke pripravljenosti
TRL 9
Kategorija velikosti kmetij
Velika, Majhna
Pridelovalni sistemi
V rastlinjaku, Na prostem/njiva
Vrste tehnik
ELISA, RNA in DNA metode (Tehnike diagnostike in detekcije)
Območje uporabe
Virusi
Ime podajetja
Rioreba
Izvorna država
All
Posebna zahteva
Potreba po posebnem kmetijskem zemljišču
Potreba po posebnem/dodatnem izobraževanju

Opis

DAS-ELISA (Double Antibody Sandwich ELISA) is a double antibody sandwich enzyme-linked immunosorbent method for rapid detection of virus infection in different crops (tomato, lettuce, onion, citrus, pepper, etc).

The test can be used for rapid detection of CTV (citrus tristeza virus) in various tissues of citrus. Field samples should be collected preferably in spring or autumn, whereas sampling during prolonged hot weather should be avoided. Samples from 3-4 different locations on the tree should be pooled and samples are homogenized 1:20 (w/v) in an extraction buffer (Garnsey & Cambra (1991).

Za več informacij
Uporabljene rastlinske vrste
  • Sadno drevje
  • Solata
  • Čebula
  • Pepper
  • Paradižnik
Možne rastlinske vrste
  • Paprika
  • Sadno drevje
  • Čebula
Države, ki so uporabljene/vključene v iskalno geslo.
  • All
Države, ki niso ustrezne
  • No Countries
Točnost

ELISA technology used

Ključna beseda
  • Pest and disease identification