DAS-ELISA (Double Antibody Sandwich ELISA) is a double antibody sandwich enzyme-linked immunosorbent method for rapid detection of virus infection in different crops (tomato, lettuce, onion, citrus, pepper, etc).
The test can be used for rapid detection of CTV (citrus tristeza virus) in various tissues of citrus. Field samples should be collected preferably in spring or autumn, whereas sampling during prolonged hot weather should be avoided. Samples from 3-4 different locations on the tree should be pooled and samples are homogenized 1:20 (w/v) in an extraction buffer (Garnsey & Cambra (1991).
ELISA technology used